Synaptopodin 2 represses cervical cancer cell growth and enhances the sensitivity of cervical cancer cells to cisplatin via Hippo pathway

Purpose: To explore the functions and regulatory mechanisms of synaptopodin-2 (SYNPO2) in cervical cancer progression.
 Methods: Normal cell lines (Ect1/E6E7) (HeLa, SiHa, C-33A, CaSki) were cultured. The pcDNA3.1 vector overexpressing SYNPO2, a negative control (vector), blank (control) transfected into HeLa SiHa cells. Protein expression from normal was examined by western blot. Cell viability proliferation evaluated cells using Counting Kit-8 colony formation assays, while migration invasion assessed wound healing Transwell respectively. apoptosis determined flow cytometry.
 Results: SYNPO2 decreased based on Gene Expression Profiling Interactive Analysis website (p < 0.05). Additionally, Kaplan–Meier Plotter showed that patients with low worse prognoses than high Subsequent investigations revealed overexpression repressed proliferation, migration, 0.01). Furthermore, enhanced 0.001) increased sensitivity to cisplatin function Hippo pathway indicate inactivated 0.05).
 Conclusion: Synaptopodin 2 represses growth enhances via pathway, thus indicating its potentials for development treatment cancer.